The amidase had apparent K(m) values of 1.2, 2.6, 3.0, 2.7, and 5.0 mM for
acrylamide, acetamide, butyramide, propionamide, and isobutyramide, respectively.
the enzyme(amidase) contains 8 mol of iron per mol of the native enzyme.
The amidase activity was enhanced by, but not dependent on Fe(2+), Ba(2+), and Cr(2+).
However, the enzyme activity was partially inhibited by Mg(2+) and totally inhibited in the presence
of Ni(2+), Hg(2+), Cu(2+), Co(2+), specific iron chelators, and thiol blocking reagents.
醯胺酶對丙烯醯胺,乙醯胺,丁醯胺,丙醯胺和異丁醯胺有明顯的K(m)值
(Vmax 及 Km 是每一個酵素極重要的性質指標,可以顯示其催化特性。
意思也就是說醯胺酶對丙烯醯胺會產生反應,會代謝丙烯醯胺。)
該酶(醯胺酶)每莫耳天然酶含有8莫耳鐵。
醯胺酶活性增強,但不依賴於Fe(鐵2+),Ba(鋇2+)和Cr(鉻2+)。
然而,酶(醯胺酶)活性被部分抑制於Mg(鎂2+),並被完全抑制於Ni(鎳2+)、 Hg(汞2+)、Cu(銅2+)、Co(鈷2+)、特定鐵螯合劑、
硫醇封閉試劑。
...(1)
The amidase activity of the EDTA-treated CwlV1 was restored by the addition of Zn2+, Mn2+, and Co2+
but not by the addition of Mg2+ and Ca2+.
EDTA處理的CwlV1的醯胺酶活性恢復
通過添加Zn2 +,Mn2 +和Co2 +
但不是通過添加Mg2 +和Ca2 +。
...(2)
Zinc is required for amidase activity but not for inhibition of T7 RNA polymerase.
鋅是醯胺酶活性所必需的
...(3)
總結以上:
一、雖然
Co(鈷2+)在(2)有助於醯胺酶活性恢復,但是在(1)卻是抑制醯胺酶活性的物質,所以Co(鈷2+)對於醯胺酶活性恢復並不可信,Co(鈷2+)似乎沒在人必須攝取的礦物質之列,應該沒有人會刻意去攝取含
Co(鈷2+)、(2)的醯胺酶活性恢復靠
通過添加Zn2+(鋅),Mn2+(錳)
,而Zn2 +,Mn2+並沒出現在
(1)的抑制醯胺酶活性的礦物質之列,所以是相當可信的;再加上(3)的敘述,更確信了鋅與醯胺酶活性有關。